Product Introduction
Taq DNA Polymerase is a thermostable recombinant DNA polymerase derived from thermophilic bacterium Thermus aquaticus. Its molecular weight is 94 kDa. Taq DNA Polymerase can amplify DNA target up to 5 kb (simple template). The elongation velocity is 2kb/min (70~75°C). It has 5' to 3' polymerase activity but lacks of 3' to 5' exonuclease activity that results in a 3'-dA overhangs PCR product.
Application
• PCR amplification of DNA fragments as long as 5 kb
• DNA labeling
• DNA sequencing
• PCR for cloning
Features
Classical and conventional Taq DNA Polymerase, 2kb/min, generates 3'-dA overhangs
Components
| Component | P1011 (500U) | P1012 (500U) | P1013 (1000U) | P1014 (1000U) | P1015 (18000U) |
| Taq DNA Polymerase (5U/μl) | 100 μl | 100 μl | 200 μl | 200 μl | 100 μl × 36 |
| 10× PCR Buffer (Mg2+ Plus)* | 1.25 ml | 1.25 ml | 1.25 ml × 2 | 1.25 ml × 2 | 1.25 ml × 36 |
| dNTPs (2.5mM each) | - | 1 ml | - | 1 ml × 2 | - |
| 6× Loading Buffer | 1 ml | 1 ml | 1 ml | 1 ml | - |
* Mg2+ Free version is also available.
Storage Condition
Store at -20°C
GDSBio has a complete product line. With PCR technology as the core, focusing on molecular biology techniques such as ordinary PCR, fluorescence quantitative PCR, NGS library preparation, nucleic acid electrophoresis, nucleic acid purification, etc.It has developed reagents for molecular biology research and diagnosis, and completed the layout of the whole industrial chain from molecular biology reagents, medical lab supplies to specialized services.
Product operation manual:P1011(mg2+free)Taq DNA Polymerase V1.2.pdf
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Taq DNA Polymerase for DNA Amplification Images
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