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General Reagents T4 Beta-glucosyltransferase

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Guangzhou Dongsheng Biotech Co., Ltd

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General Reagents T4 Beta-glucosyltransferase

Name: General Reagents T4 Betaglucosyltransferase
Brand: GDSBio
Price: 207 USD

Brand Name : GDSBio

Model Number : E1020

Certification : /

Place of Origin : China

MOQ : 500 U

Price : USD 207-230/500 U

Payment Terms : L/C, D/A, D/P, T/T, Western Union, MoneyGram

Supply Ability : 100 Bag/Bags Per Day

Delivery Time : 8 work days

Packaging Details : small package or bulk distribute or OEM

Product Name : T4 beta-glucosyltransferase

Cat. No./Spec. : E1020-A/500 U

Appearance : colourless

Application : Site-specific detection of 5-hmC

Classification : General Reagents

Grade : molecular biology

Logo Printing : With Logo Printing

Transport Package : Packing

Production Capacity : 100 Bag/Bags Per Day

Storage Conditions : Store at -20°C

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T4 β-glucosyltransferase

Cat. No./Spec.: E1020-A/500 U
Concentration: 5 U/µL

Product Description
T4 β-glucosyltransferase (T4 BGT) is capable of transferring the glucosyl group from uridine diphosphate glucose (UDP-glucose) to the 5-hydroxymethylcytosine (5-hmC) residues within double-stranded DNA, resulting in the formation of β
-glucosyl-5-hydroxymethylcytosine. This enzyme is formulated for rapid reaction times without compromising the efficiency of the reaction. It can glucosylate 5-hmC on 1 μg of DNA in 15 minutes at 37°C.

Storage Condition & Shelf Life
Store at -20°C.

Unit Definition
A unit is defined as the amount of enzyme required to protect 0.5 μg of fully 5-hydroxymethylated 1095bp PCR fragment from digestion by MunI within 1 hour at 37°C in a 50 μL volume of the recommended reaction buffer.

Features
- Specificity - Selectively transfers glucose to the hydroxymethyl group of 5-hmC.
- Speed - Completes the glucosylation of 1 μg DNA in 15 minutes.
- Convenience - Comes with optimized buffer and UDP-glucose included.

Scope of Application
- Site-specific detection of 5-hmC.
- Enrichment of DNA containing 5-hmC.
- Labeling of 5-hmC residues using radioactive UDP-glucose donor.

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T4 beta-glucosyltransferase

General Reagents T4 Beta-glucosyltransferase

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